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vanadium chloro peroxidase project started in 1991 Vanadium chloroperoxidase (VCPO) from the fungus Curvularia inaequalis has also been a topic of research in the group for a long time. This enzyme has been characterized in detail using a variety of biophysical techniques. Its crystal structure has been determined and the enzyme has been successfully expressed both in Saccharomyces cerevisia (yeast) and in E. coli. VCPO catalyzes the two-electron oxidation of both bromide and chloride to hypobromous acid (HOBr) and hypochlorous acid (HOCl), respectively. Our first directed evolution work successfully resulted in an enzyme mutant with a much higher brominating activity at slightly alkaline pH (pH 8).

References:
R. Renirie, C. Pierlot , J.-M. Aubry, A. F. Hartog, H. E. Schoemaker, P. L. Alsters and R.Wever.
Vanadium chloroperoxidase as a catalyst for hydrogen peroxide disproportionation to singlet oxygen in mildly acidic aqueous environment.
Adv. Synth. Catal. 345 (2003) 849-858

N. Tanaka, Z. Hasan and R. Wever.
Kinetic characterization of active site mutants Ser402Ala and Phe397His of vanadium chloroperoxidase from the fungus Curvularia inaequalis.
Inorg. Chim. Acta 356 (2003) 288-296

N. Tanaka and R. Wever.
Inhibition of vanadium chloroperoxidase from the fungus Curvularia inaequalis by hydroxylamine, hydrazine and azide and inactivation by phosphate.
J. Inorg. Biochem. 98 (2004) 625-631.

Z. Hasan, R. Renirie, R. Kerkman, A. F. Hartog and R. Wever.
Laboratory-evolved vanadium chloroperoxidase exhibits 100-fold higher halogenating activity at alkaline pH.
J. Biol. Chem. 281 (2006) 9738-9744

N. Pooransingh-Margolis, R. Renirie, Z. Hasan Z, R. Wever, A.J. Vega, T. Polenova.
51V solid-state magic angle spinning NMR spectroscopy of vanadium chloroperoxidase.
J. Am. Chem. Soc. 128 (2006) 5190-5208

R. Wever. R. Renirie and Z. Hasan.
Vanadium in Biology.
In Encyclopedia Inorg. Chem. Section Bioinorganic Chemistry (R.A Scott, Section editor), 2006,
www.wiley.com/eic2

Renirie R, Dewilde A, Pierlot C, Wever R, Hober D, Aubry JM.
Bactericidal and virucidal activity of the alkalophilic P395D/L241V/T343A mutant of vanadium chloroperoxidase.
J Appl Microbiol. 2008 Jul;105(1):264-70.
PUBMED

de Macedo-Ribeiro S, Renirie R, Wever R, Messerschmidt A.
Crystal structure of a trapped phosphate intermediate in vanadium apochloroperoxidase catalyzing a dephosphorylation reaction.
Biochemistry. 2008 Jan 22;47(3):929-34.
PUBMED

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